Copper(II) partially protects three histidine residues and the N‐terminus of amyloid‐β peptide from diethyl pyrocarbonate (DEPC) modification
نویسندگان
چکیده
منابع مشابه
Histidine modification with diethyl pyrocarbonate shows heterogeneity of benzodiazepine receptors.
The effect of diethyl pyrocarbonate modification of histidine on the specific binding of [3H]diazepam and its enhancement with muscimol and (+/-)-pentobarbital was investigated. Diethyl pyrocarbonate treatment produced a dose-related inhibition of specific [3H]diazepam binding to rat brain membranes with a maximal inhibition of approximately 40% at 1 mM. Scatchard analysis of the binding data s...
متن کاملStabilization of tumor antigens by chemical modification with diethyl pyrocarbonate.
C3H mice were immunized with MH134 tumors modified with two different chemicals, DEPC and TNBS, and the tumor-neutralizing activities of the spleen cells were examined after separating the cells by a Nylon-wool column, plastic dish, or treatment with specific antibodies (anti-mouse IgG and anti-mouse theta sera). The effector cells induced in mice by immunization with TNBS-treated tumors were T...
متن کاملModification of Histidine Residues of Photosystem II by Diethyl Pyrocarbonate Inhibits the Electron Transfer between the Primary (QA) and Secondary (QB) Quinone Acceptors
The effect o f diethyl pyrocarbonate (DEPC) on the photosynthetic electron transport was investigated in isolated spinach thylakoids by partial electron transport rate and thermolumi nescence measurements. Incubation o f thylakoids at pH 6.5 with 5 mM DEPC for 15 min re sulted in a considerable inhibition o f electron transport from water to dichlorophenolindophenol. The inhibition was only p...
متن کاملEffect of diethyl pyrocarbonate modification on the calcium binding mechanism of the sarcoplasmic reticulum ATPase.
Diethyl pyrocarbonate was used to modify histidyl residues on the sarcoplasmic reticulum ATPase. Difference spectra of the N-carbethoxyhistidyl derivative indicated that most all the histidyl residues on the enzyme had been modified. These residues could be divided into two populations on the basis of their reaction rate with the reagent. It could then be shown that enzyme inhibition followed m...
متن کاملCircular dichroism studies of diethyl pyrocarbonate-modified histidine in hen egg white lysozyme.
The single histidine residue (His-15) in hen egg white lysozyme (EC 3.2.1.17) was chemically modified by diethyl pyrocarbonate (DEPC) to form exclusively the mono-N-carbethoxyimidazole adduct (second order rate constant of 252 +/- 16 M-1 min-1). Irreversible biscarbethoxylation of the His-15 imidazole ring by DEPC was observed when lysozyme was pretreated with 2-mercaptoethanol (2-ME), 2-ME plu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Open Bio
سال: 2020
ISSN: 2211-5463,2211-5463
DOI: 10.1002/2211-5463.12857